Cell cycle arrest and apoptosis induced by searsia Rhemanniana extracts in du145 prostate cancer cells

Title : Cell cycle arrest and apoptosis induced by searsia Rhemanniana extracts in du145 prostate cancer cells

Abstract:

This research examined the impacts of Searsia rhemanniana extracts on the progression of the cell cycle and apoptosis in DU145 prostate cancer cells, utilizing Melphalan as a reference chemotherapy drug. Different mechanistic reactions were noted among the extracts. Melphalan caused G2/M arrest and apoptosis, aligning with cytotoxicity mediated by DNA damage. Bark methanolic and leaf aqueous extracts induced significant apoptosis without any cell cycle arrest beforehand, indicating a mechanism that is independent of checkpoints. In comparison, dichloromethane extracts from bark and root induced mitotic (M-phase) arrest, suggesting spindle disruption. The aerial methanolic extract exhibited a Melphalan-like pattern, causing G2 arrest and apoptosis, whereas certain leaf extracts demonstrated minimal effects. In summary, S. rhemanniana exhibits various anticancer effects that are specific to different mechanisms, highlighting its potential as a source of new therapeutic agents for prostate cancer.

Biography:

Ms. D.E. Rampana-Moleleki pursued Biomedical Technology at the Central University of Technology in Free State, South Africa, and earned a National Diploma in 2006. She subsequently became part of the research team led by Prof. Makhoahle at the same university, within the Centre for Quality Health and Living (CQHL). In 2021, she obtained her master’s degree from the same university. At present, she is working on her PhD at the Central University of Technology, Free State, while serving as a lecturer and researcher. Ms. Rampana has authored research papers in SCI(E) journals, making a substantial impact on her discipline.